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INSL 3 C-Peptide (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-035-48 100 l $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat brain and testis tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Insulin-like 3 C Peptide/Insulin-like 3 Prepro (58-105) (Human) Serum (Catalog No: H-035-48)
 Optimal Dilution 1:50-200 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec

Catalog #H-035-48
Standard Size100 l
SequencePro - Ala - Ala - Gly - Gly - Asp - Arg - Glu - Leu - Leu - Gln - Trp - Leu - Glu - Arg - Arg - His - Leu - Leu - His - Gly - Leu - Val - Ala - Asp - Ser - Asn - Leu - Thr - Leu - Gly - Pro - Gly - Leu - Gln - Pro - Leu - Pro - Gln - Thr - Ser - His - His - His - Arg - His - His - Arg
SpeciesHuman
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50l of distilled water for the equivalent of undiluted antiserum, immediately before use. Reconstituted antibody can also be aliquotted (5~10l) and stored frozen at
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