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G-028-28A
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RBP4 (Human) - Purified IgG Antibody
Catalog#
Standard size
Price
Order
G-028-28A
100 µg
$275.00
Retinol Binding Protein 4
RBP-4
Catalog #
G-028-28A
Standard Size
100 µg
Species
Human
Host
Rabbit
Immunogen
Purified human RBP-4
Storage Condition
For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Contents
Each vial contains 100µl of rabbit IgG 100mM NaCI, 15 mM NaN
3
. The RBP-4 (Human) can be detected by this antibody at 1:10K (0.1 µl/ml) in ELISA We recommend that each laboratory determine an optimum titer for use in its particular assay system.
Cross Reactivity
This antibody reacts with a circulatory RBP-4 in human serum or plasma.
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Protocol for Immunohistochemistry
Tissue Sample
human placenta & rat liver tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Retinol-Binding Protein-4 (Human) (Catalog No.:G-028-28-B)
Optimal Dilution
1:250-500 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection system
HRP
Substrate
DAB (Sigma)
Counterstained
Hematoxylin
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