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EM-66 / Secretogranin II (219-284) (Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-047-69 100 l $450.00

Image    
Western Blot Analysis of Secretogranin II in rat brain tissue
 Tissue Sample Rat brain tissue
 Electrophoresis 10% SDS-PAGE gel, reducing condition
 Denatured sample  100'C for 5 min
 Positive control Rat Secretogranin II peptide
 Blocking 1% BSA in TBST
 Primary Antibody Rabbit Anti-EM-66/Secretogranin II (219-284) (Rat)  Serum (Catalog No.:H-047-69)
 Optimal Dilution 1:200 (overnight at cold room)
 Secondary Antibody Anti-Rabbit whole IgG, HRP conjugated
 Positive Band  ~ 68 KDa
 Substrate ECL
Catalog #H-047-69
Standard Size100 l
SequenceGlu - Arg - Val - Asp - Glu - Glu - Gln - Lys - Leu - Tyr - Thr - Asp - Asp - Glu - Asp - Asp - Val - Tyr - Lys - Thr - Asn - Asn - Ile - Ala - Tyr - Glu - Asp - Val - Val - Gly - Gly - Glu - Asp - Trp - Ser - Pro - Met - Glu - Glu - Lys - Ile - Glu - Thr - Gln - Thr - Gln - Glu - Glu - Val - Arg - Asp - Ser - Lys - Glu - Asn - Thr - Glu - Lys - Asn - Glu - Gln - Ile - Asn - Glu - Glu - Met
SpeciesRat
HostRabbit
ReconstitutionReconstitute with 100 l distill water.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorDirect ELISA: 1: 3200
Western blot : 1:200
Cross ReactivityThis antibody is crossreactivity with rat EM-66 peptide or Secretogranin II (219-284) at 100%. It was tested in western blot in rat brain tissues.
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