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H-019-27
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Stresscopin Related Peptide (SRP) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-019-27
50 µl
$450.00
Stresscopin, Stresscopin Related Peptides & Wide Range RIA Kit
Urocortin II---A New Obesity Peptide
Prepro-Stresscopin Related Peptide (SRP) Sequence
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Human pancreas tissue and Rat brain tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Stresscopin-related Peptide (SRP)(Human) Antiserum (Catalog No.:H-019-27)
Optimal Dilution
1:500 (overnight at 4 oC)
Secondary Antibody
Goat anti-Rabbit IgG, HRP conjugated
Amplification
ABC (Vector) (1:400, 30 min)
Substrate
DAB (Sigma), 10"
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-019-27
Standard Size
50 µl
Sequence
His - Pro - Gly - Ser - Arg - Ile - Val - Leu - Ser - Leu - Asp - Val - Pro - Ile - Gly - Leu - Leu - Gln - Ile - Leu - Leu - Glu - Gln - Ala - Arg - Ala - Arg - Ala - Ala - Arg - Glu - Gln - Ala - Thr - Thr - Asn - Ala - Arg - Ile - Leu - Ala - Arg - Val - NH2
Species
Human
Host
Rabbit
Immunogen
Stresscopin Related Peptide (SRP)(Human)
Reconstitution
For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of Rabbit Anti-Stresscopin Related Peptide (SRP) (Human) Serum in the lyophilized form.
Recommended Dilution Factor
Direct ELISA: 1: 3200
Immunohistochemistry: 1:500
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