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Endothelin-1 (ET-1) (Human, Rat, Mouse, Porcine, Bovine, Canine, Rabbit, Monkey) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-023-01 50 l $175.00

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Protocol for Immunohistochemistry
 Tissue SampleRat Kidney Tissue and Mouse Lung Tissue
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentTarget retrieval 25 min (Steam)
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-Endothelin-1  Serum (Catalog No.: H-023-01)

 Optimal Dilution

1:200, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

ABC (Vector) (1:400, 30 min)

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-023-01
Standard Size50 l
SequenceCys - Ser - Cys - Ser - Ser - Leu - Met - Asp - Lys - Glu - Cys - Val - Tyr - Phe - Cys - His - Leu - Asp - Ile - Ile - Trp

Disulfide Bridge: Cys1 - Cys15 and Cys3 - Cys11
SpeciesHuman, Mouse, Porcine, Canine, Rat, Bovine
Specific SpecieRabbit, Monkey
HostRabbit
ImmunogenEndothelin-1 (ET-1) (Human, Rat, Mouse, Porcine, Bovine, Canine)
ReconstitutionFor best and reproducible results, reconstitute with 50l of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine) Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC: 1:500
Immunohistochemistry - 1:200.
Cross Reactivity
Peptide % Cross-reactivity  
Endothelin-1 (Human, Rat, Mouse, Porcine, Bovine, Canine, Rabbit, Monkey)  100
Big Endothelin-2 (Human)70
Big Endothelin-1(Human)50
Endothelin-3 (Human, Rat, Porcine) 28
Endothelin-2 (Human, Canine) 3.5
Big Endothelin-3 (Human)  0.2
Angiotensin II (Human) 0
[Arg8]-Vasopressin 0
Alphabet_E_
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