Endothelin-1, Big (Human) - Antibody for Immunohistochemistry

Catalog #

H-023-10

Standard Size

50 µl

Sequence

Cys - Ser - Cys - Ser - Ser - Leu - Met - Asp - Lys - Glu - Cys - Val - Tyr - Phe - Cys - His - Leu - Asp - Ile - Ile - Trp - Val - Asn - Thr - Pro - Glu - His - Val - Val - Pro - Tyr - Gly - Leu - Gly - Ser - Pro - Arg - Ser

Disulfide Bridge: Cys1 - Cys15 and Cys3 - Cys11

Species

Human

Host

Rabbit

Immunogen

Endothelin-1 (ET-1) Big (Human)

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- Big Endothelin-1 (Human) Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500
Immunohistochemistry - 1:1000. (J Exp Biol. 2007 Dec;210(Pt 24):4286-97.)

Cross Reactivity

*Peptide*	*% Cross-reactivity*
Big Endothelin 1 (Human)	100
Big Endothelin 1 22-38 (Human)	100
Big Endothelin 22-39 (Porcine)	2.0
Big Endothelin (Porcine)	1.0
Endothelin 1 (Human, Rat, Porcine, Mouse, Canine, Bovine)	0
Endothelin 2 (Human, Canine)	0
Endothelin 3 (Human, Rat)	0
BNP-32 (Human)	0
Sarafotoxin S6b	0

Alphabet_E_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Hyndman et al. Endothelin and endothelin converting enzyme-1 in the fish gill: evolutionary and physiological perspectives
J Exp Biol. 2007 Dec;210(Pt 24):4286-97.

Gray et al. Evaluation of the mouse prostate as a suitable model for the study of human prostate function.
J Pharmacol Toxicol Methods. 2005 Jan-Feb;51(1):41-50.