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Ghrelin (Rat, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-031-31 100 l $450.00

Image

Protocol for Western Blot Analysis: 
 Sample Rat stomach tissue homogenate  
 Electrophoresis 15% Tricine-PAGE gel, reducing condition
 Sample Buffer Tricine sample buffer (BioRad)
 Positive Control Ghrelin (Rat, Mouse)
 Transfer Membrane 0.2 µm PVDF
 Blocking 1% BSA in TBST
 Primary Antibody Rabbit Anti-Ghrelin (Rat, Mouse) Antibody (Catalog No.:H-031-31)
 Optimal Dilution 1:400 ~ 500 (overnight at cold room)
 Secondary Antibody Anti-Rabbit whole IgG , HRP conjugated (1: 12500)
 Substrate ECL
 Positive Band

 ~ 13 KDa (prepro-Ghrelin, 118 aa)

 Protocol for Immunohistochemistry

 Tissue Sample Rat stomach tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Ghrelin (Rat, Mouse) Antibody (Catalog No.:H-031-31)
 Optimal Dilution 1:100 ~200 (overnight hour at 4oC)
 Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
 Substrate DAB (Sigma), 10"
 Counterstained Hematoxylin, 30 Sec




Ghrelin-immunoreactive (IR) cells in fetal and neonatal pancreas were stained by Phoenix Ghrelin (Rat) Antibody (H-031-31) (1:800). Dr. Frank Sundler & Dr. N. Wierup Neuroendocrine Cell Biology, University of Lund,  Sweden.

Catalog #H-031-31
Standard Size100 l
SequenceGly - Ser - Ser (Octanoyl) - Phe - Leu - Ser - Pro - Glu - His - Gln - Lys - Ala - Gln - Gln - Arg - Lys - Glu - Ser - Lys - Lys - Pro - Pro - Ala - Lys - Leu - Gln - Pro - Arg
SpeciesRat, Mouse
HostRabbit
ReconstitutionReconstitute with 100 l of distilled water.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti-Ghrelin (Rat, Mouse) Serum in the lyophilized form.
Recommended Dilution FactorDirect ELISA: 1: 3200
Western blot: 1:400 ~ 500
Cross Reactivity
Peptide % Cross-reactivity 
 Ghrelin (Rat, Mouse) 100
 Ghrelin (Human) 100
 Ghrelin (Des-Octanoyl-Ser3) (Human) 100
 Ghrelin (Des-Octanoyl-Ser3) (Rat, Mouse)  100
 Ghrelin (17-28) (Human, Rat) 100
 Ghrelin (Canine) 100
 Ghrelin C-terminal Hexapeptide (Human Rat) 3
 Ghrelin (Porcine) 0
 Ghrelin (1-14) (Human)  0
 Ghrelin (1-5)-NH2 (Human, Rat)  0
 Ghrelin (1-5)-NH2 (Des-Octanyl3) (Human Rat) 0
 Ghrelin (1-14) (Des-Octanoyl3) (Human)  0
 Secretin (Human) 0
 VIP (Human, Rat, Porcine)  0
 Prolactin-Releasing Peptide-31 (Rat)  0
 Prolactin-Releasing Peptide-31 (Human) 0
 Galanin (Rat)  0
 Galanin (Human) 0
 GHRF (Rat)  0
 GHRF (Human) 0
 NPY (Human, Rat)  0
 Orexin A (Human, Rat, Mouse) 0
 Orexin B (Human) 0
Alphabet_G_
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