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Defensin 1, beta (Human) (synthetic) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-072-53 100 l $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat & Mouse lung, Human lung cancer tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No Primary Antibody
 Pretreatment N/A
 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Beta-Defensin I (H) Antiserum (Catalog No.: H-072-53)
 Optimal Dilution 1:500 , 1 hour at RT
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  Streptavidin-HRP (Vector), 1:400, 30 min
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-072-53
Standard Size100 l
SequenceAsp - His - Tyr - Asn - Cys - Val - Ser - Ser - Gly - Gly - Gln - Cys - Lys - Tyr - Ser - Ala - Cys - Pro - Ile - Phe - Thr - Lys - Ile - Gln - Gly - Thr - Cys - Tyr - Arg - Gly - Lys - Ala - Lys - Cys - Cys - Lys Disulfide bridges between Cys1 & Cys5, Cys2 & Cys4 and Cys3 & Cys6
SpeciesHuman
HostRabbit
ImmunogenBeacon (47-73)
ReconstitutionRehydrate the lypholized antiserum with 100l deionized water, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti- β-Defensin 1 (Human) Serum in the lyophilized form.
Recommended Dilution FactorIndirect ELISA 1: 6400
Immunohistochemistry 1: 250~ 500
Cross ReactivityThis antiserum exhibits 100% cross-reactivity with beacon (47-73) of Psammomys obesus, and does not cross-react with following peptides: angiotensin I (human), cholecystokinin octapeptide (26-33 non-sulfated form), neurotensin, galanin (porcine), motilin (porcine), g3-MSH, somatostatin-28, arginine-vasopressin, calcitonin (rat), neuropeptide Y (human, rat), or glucagons-like peptide-1 (7-37).
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