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H-035-40
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INSL 5 (Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-035-40
50 µl
$450.00
Relaxin & Relaxin-Related Peptides
Insulin-like 5 Peptide (INSL 5)
Relaxin Evolution
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Mouse brain or rat testis tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody (Pre-immuno serum)
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-INSL 5 (Mouse) Antiserum (Catalog No.:H-035-40)
Optimal Dilution
1:100~200 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-035-40
Standard Size
50 µl
Sequence
A - Chain DLQALCCREGCSMKELSTLC B - Chain SRQTVKLCGLDYVRTVIYICASSRW
Species
Mouse
Host
Rabbit
Reconstitution
Lyophilized antiserum are stable for twelve months from date of receipt when stored at -20Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
Dun et al. Insulin-Like Peptide 5: Expression in the Mouse Brain and Mobilization of Calcium
Endocrinology. 2006 Jul;147(7):3243-8.
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