Assay Protocol (PDF)
Note: Read this protocol thoroughly prior to performing the assay!Preliminary tests must be done to determine the dilution factor of samples for optimal results. Un-extracted human plasma sample of normal subject should be diluted to 1:1000 - 1:2000 using 1x assay buffer. It is up to the discretion of each laboratory to determine appropriate conditions for this assay.
Links to publications that used this kit:
Wu et al. Serum retinol binding protein 4 and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus.Diabetes Res Clin Pract. 2008 Feb;79(2):185-90.Hu et al. Effect of RBP4 gene variants on circulating RBP4 concentration and type 2 diabetes in a Chinese population.Diabet Med. 2008 Jan;25(1):11-8.
Shea et al. Serum retinol-binding protein 4 concentrations in response to shortterm overfeeding in normal-weight, overweight, and obese menAm J Clin Nutr. 2007 Nov;86(5):1310-5.
Weiping et al. Elevated serum RBP4 is associated with insulin resistance in women with polycystic ovary syndrome.Endocrine. 2006 Dec;30(3):283-7.
