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Visfatin / PBEF Isoform I, His (Rec.) (Mouse) - Purified IgG Antibody

Catalog# Standard size Price Order
G-003-91 100 µg $450.00

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Protocol for Immunohistochemistry:
 Tissue Sample Rat or Mouse Visceral white fat tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Visfatin Isoform I, His, (Rec.) (Mouse) (Catalog No.:G-003-91)
 Optimal Dilution 1:1000 ~ 2000 (Overnight at 4oC)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification
 ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (sigma), 30min
 Counterstained Hematoxylin, 30Sec
Catalog #G-003-91
Standard Size100 µg
SequenceMouse PBEF Isoform I, 6 x His on N - Terminal.
SpeciesMouse
HostRabbit
ReconstitutionReconstitute with 100 ul distill water or PBS.
Storage ConditionFor optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.
Content100 ul NET Rabbit serum in Lyophilized powder without NaN3 and carry protein.
Recommended Dilution FactorIndirect ELISA: 1: 6400
Immunohistochemistry:1: 1000~2000
Cross ReactivityIt is cross reactive with Mouse Visfatin/PBEF Isoform I, His, (Rec.). It was tested in immunohistochemistry in mouse and rat visceral white fat tissues. Not yet tested in other species.
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