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H-035-56
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INSL 4 Prepro-C-Peptide (64-110) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-035-56
100 µl
$295.00
Relaxin & Relaxin-Related Peptides
Insulin-like 4 Peptide (INSL 4)
Relaxin Evolution
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Human Placenta tissue
Fixative
4% Paraformaldehyde 8 hours
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
10% Normal Goat Serum
Primary Antibody
Rabbit Anti-INSL4, C Peptide (Human) Antibody (Catalog No.:H-035-56)
Optimal Dilution
1:100 (1 hour at 37°C and overnight 4°C
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (Vector Laboratory) (37°C 60 min)
Amplification
ABC (Vector) (30 min)
Detection system
HRP
Substrate
DAB (Vector), 2 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-035-56
Standard Size
100 µl
Sequence
Pro - Lys - Glu - Met - Val - Ser - Thr - Ser - Asn - Asn - Lys - Asp - Gly - Gln - Ala - Leu - Gly - Thr - Thr - Ser - Glu - Phe - Ile - Pro - Asn - Leu - Ser - Pro - Glu - Leu - Lys - Lys - Pro - Leu - Ser - Glu - Gly - Gln - Pro - Ser - Leu - Lys - Lys - Ile - Ile - Leu - Ser
Species
Human
Host
Rabbit
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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