INSL 4 Prepro-C-Peptide (64-110) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human Placenta tissue
Fixative	4% Paraformaldehyde 8 hours
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Intact
Blocking	10% Normal Goat Serum
Primary Antibody	Rabbit Anti-INSL4, C Peptide (Human) Antibody (Catalog No.:H-035-56)
Optimal Dilution	1:100 (1 hour at 37°C and overnight 4°C
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (Vector Laboratory) (37°C 60 min)
Amplification	ABC (Vector) (30 min)
Detection system	HRP
Substrate	DAB (Vector), 2 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-035-56

Standard Size

100 µl

Sequence

Pro - Lys - Glu - Met - Val - Ser - Thr - Ser - Asn - Asn - Lys - Asp - Gly - Gln - Ala - Leu - Gly - Thr - Thr - Ser - Glu - Phe - Ile - Pro - Asn - Leu - Ser - Pro - Glu - Leu - Lys - Lys - Pro - Leu - Ser - Glu - Gly - Gln - Pro - Ser - Leu - Lys - Lys - Ile - Ile - Leu - Ser

Species

Human

Host

Rabbit

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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