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H-059-08
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PYY (3-36)-Gly (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-059-08
100 µl
$350.00
PYY
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Image
Protocol for Immunohistochemistry:
Tissue Sample
Human Colon
Fixative
10% formalin
Embedding
Paraffin
Negative Control
No primary antibody
Pretreatment
N/A
Blocking
3% H
2
O
2
, 2% Normal Goat Serum
Primary Antibody
Rabbit Anti-PYY (3-36)-Gly (H) Antiserum (Catalog No.: H-059-08)
Optimal Dilution
1:500, 1 hour at RT
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 sec
Catalog #
H-059-08
Standard Size
100 µl
Sequence
Ile - Lys - Pro - Glu - Ala - Pro - Gly - Glu - Asp - Ala - Ser - Pro - Glu - Glu - Leu - Asn - Arg - Tyr - Tyr - Ala - Ser - Leu - Arg - His - Tyr - Leu - Asn - Leu - Val - Thr - Arg - Gln - Arg - Tyr - Gly
Species
Human
Host
Rabbit
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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