PYY Antibodies

Peptide YY (PYY) Antibodies

Mapping in Rat Colon by PYY (Human) Antibody (H-059-01)

Tissue Sample	Rat colon tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	Pre-immuno serum
Pretreatment	N/A
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (Human) Serum (Catalog No.:H-059-01)
Optimal Dilution	1:200~500 (1hour at RT)
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Mapping in Mouse and Rat Intestine tissue by PYY (3-36) (Human) Antibody (H-059-02)

Tissue Sample	Mouse and rat intestine tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (3-36) (Human) Serum (Catalog No.:H-059-02)
Optimal Dilution	1:250~500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	ABC (Vector) (1:400, 30 min)
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (1-36)-Gly Antibody (H-059-06)

Tissue Sample	Human Colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H2O2, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (1-36)-Gly (H) Antiserum (Catalog No.: H-059-06)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-36)-Gly (Human) Antibody (H-059-08)

Tissue Sample	Human Colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H2O2, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (3-36)-Gly (H) Antiserum (Catalog No.: H-059-08)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-36)-Gly (Rat, Mouse, Porcine, Canine) Antibody (H-059-09)

Tissue Sample	Human Colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H2O2, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (3-36)-Gly (R,M,C,P) Antiserum (Catalog No.: H-059-09)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-19) (Human) Antibody (H-059-16)

Tissue Sample	Human Colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H2O2, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (3-19) (H) Antibody (Catalog No.: H-059-16)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-19)-Gly Antibody (H-059-17)

Tissue Sample	Human Colon
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	N/A
Blocking	3% H2O2, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-PYY (3-19)-Gly (H) Antibody (Catalog No.: H-059-17)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection System	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Western Blot Analysis of PYY in rat intestine tissue by PYY 3-36 (Human) Antibody (H-059-02)

Tissue Sample	Rat intestine tissue homogenate
Fixative	10% formalin
Electrophoresis	15% Tricine-PAGE gel
Negative Control	No primary antibody
Blocking	1% BSA in TBST or 5% dry milk in TBST
Primary Antibody	Rabbit Anti-PYY (3-36) (Human) Serum (Catalog No.:H-059-02)
Optimal Dilution	1:200 ( 5 ug /ml) (4 hour at RT, or overnight at 40C)
Secondary Antibody	Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection System	HRP
Substrate	SuperSignal Chemiluminescent Substrate