中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站 www.phoenixpeptide.com
PHOENIX PHARMACEUTICALS, INC. TOP HOME PAGE
Top Catalog English Version | My Account | 联系我们 | China



 多肽



 标记多肽 



 多肽激素文库



 抗体 



 免疫试剂盒 



 生物标志物阵列 



 多肽样品检测



 自定义肽链合成及GMP



 产品目录索取



 样品准备



 提问和解答


Peptide YY (PYY) Antibodies

Mapping in Rat Colon by PYY (Human) Antibody (H-059-01)

Tissue Sample

Rat colon tissue

Fixative

10% formalin

Embedding

Paraffin

Negative Control

Pre-immuno serum

Pretreatment

N/A

Blocking

2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (Human) Serum (Catalog No.:H-059-01)

Optimal Dilution

1:200~500 (1hour at RT)

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

ABC (Vector) (1:400, 30 min)

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Mapping in Mouse and Rat Intestine tissue by PYY (3-36) (Human) Antibody (H-059-02)

Tissue Sample

Mouse and rat intestine tissue

Fixative

10% formalin

Embedding

Paraffin

Negative Control

Pre-immuno serum

Pretreatment

Intact

Blocking

2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (3-36) (Human) Serum (Catalog No.:H-059-02)

Optimal Dilution

1:250~500, 1 hour at RT

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

ABC (Vector) (1:400, 30 min)

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (1-36)-Gly Antibody (H-059-06)

Tissue Sample

Human Colon

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (1-36)-Gly (H) Antiserum (Catalog No.: H-059-06)

Optimal Dilution

1:500, 1 hour at RT

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-36)-Gly (Human) Antibody (H-059-08)

Tissue Sample

Human Colon

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (3-36)-Gly (H) Antiserum (Catalog No.: H-059-08)

Optimal Dilution

1:500, 1 hour at RT

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-36)-Gly (Rat, Mouse, Porcine, Canine) Antibody (H-059-09)

Tissue Sample

Human Colon

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (3-36)-Gly (R,M,C,P) Antiserum (Catalog No.: H-059-09)

Optimal Dilution

1:500, 1 hour at RT

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-19) (Human) Antibody (H-059-16)

Tissue Sample

Human Colon

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (3-19) (H) Antibody (Catalog No.: H-059-16)

Optimal Dilution

1:500, 1 hour at RT

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Mapping in Human Colon Tissue by PYY (3-19)-Gly Antibody (H-059-17)

Tissue Sample

Human Colon

Fixative

10% formalin

Embedding

Paraffin

Negative Control

No primary antibody

Pretreatment

N/A

Blocking

3% H2O2, 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-PYY (3-19)-Gly (H) Antibody (Catalog No.: H-059-17)

Optimal Dilution

1:500, 1 hour at RT

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

Detection System

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 sec

Western Blot Analysis of PYY in rat intestine tissue by PYY 3-36 (Human) Antibody (H-059-02)

Tissue Sample

 Rat intestine tissue homogenate

Fixative

10% formalin

Electrophoresis

15% Tricine-PAGE gel

Negative Control

No primary antibody

Blocking

1% BSA in TBST or 5% dry milk in TBST

Primary Antibody

Rabbit Anti-PYY (3-36) (Human) Serum (Catalog No.:H-059-02)

Optimal Dilution

1:200 ( 5 ug /ml) (4 hour at RT, or overnight at 40C)

Secondary Antibody

Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)

Detection System

HRP

Substrate

SuperSignal Chemiluminescent Substrate

%pyy%


分类搜索
关键字搜索
按字母搜索
A B C D E F G H I J K L M N
O P Q R S T U V W X Y Z

Copyright 2019 PHOENIX BIOTECH