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Musclin / Osteocrin (Ostn) (83-133) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-68 100 µl $350.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Human skeletal muscle tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Anti-Musclin / Osteocrin (83-133) (Human) antibody (Catalog   No.:H-028-68)
 Optimal Dilution1:500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector)(1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-028-68
FamilyOsteocrin
Standard Size100 µl
SequenceSer - Phe - Ser - Gly - Phe - Gly - Ser - Pro - Leu - Asp - Arg - Leu - Ser - Ala - Gly - Ser - Val - Asp - His - Lys - Gly - Lys - Gln - Arg - Lys - Val - Val - Asp - His - Pro - Lys - Arg - Arg - Phe - Gly - Ile - Pro - Met - Asp - Arg - Ile - Gly - Arg - Asn - Arg - Leu - Ser - Asn - Ser - Arg - Gly
SpeciesHuman
HostRabbit
ImmunogenMusclin / Osteocrin (Ostn) (83-133)(Human)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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