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Secreted Frizzled-related Protein 2 / SFRP 2 (25-50) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-006-10 100 l $295.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat/Mouse Visceral Fat
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-sFRP-2 (25-50) Antiserum (Catalog No: H-006-10)
 Optimal Dilution 1:500 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-006-10
FamilysFRP
Standard Size100 l
SequenceGln - Gly - Gly - Glu - Leu - Val - Ile - Thr - Ser - Val - Lys - Arg - Trp - Gln - Lys - Gly - Gln - Arg - Glu - Phe - Lys - Arg - Ile - Ser - Arg - Ser - Ile - Arg - Lys - Leu - Gln - Cys
SpeciesHuman
Hostrabbit
ImmunogensFRP-2 (25-50) (Human)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_s_

%006-11%;%006-10%

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