Protocol for Immunohistochemistry:
Tissue Sample
Human placenta tissue
Fixative
10% formalin
Embedding
Paraffin
Negative Control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-VASH1 Protein (177-204) (Human) Antiserum (Catalog No.:H-002-95)
Optimal Dilution
1:500 (overnight at 4 oC)
Secondary Antibody
Goat anti-Rabbit IgG, HRP conjugated
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec