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H-071-24
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Urotensin II, Prepro (21-40) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-071-24
100 µl
$450.00
Urotensin II
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Human placenta
Fixative
10% Formalin
Embedding
Paraffin
Negative control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Urotensin II, Prepro (21-40) (Human) Serum (Catalog No.:H-071-24)
Optimal Dilution
1:500 (over night at 4°C)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-071-24
Standard Size
100 µl
Sequence
Leu - Pro - Leu - Leu - Asp - Ser - Arg - Glu - Ile - Ser - Phe - Gln - Leu - Ser - Ala - Pro - His - Glu - Asp - Ala
Species
Human
Reconstitution
Reconstitute with 100 ul distilled water.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution Factor
1: 500 (immunohistochemistry)
1: 3200 (indirect ELISA)
Cross Reactivity
This antibody is crossreactivity with human Urotensin II, Prepro (21-40). It was tested in human placenta tissues in immunohistochemistry
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