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Angiopoietin-related Growth Factor (AGF) / Angiopoietin-like 6 (215-230) (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-92 100 µl $450.00

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Protocol for Immunohistochemistry
 Tissue SampleRat liver Tissue
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-AGF/ANGPTL6 (215-350) (Mouse) serum (Catalog No.: H-028-92)

 Optimal Dilution

1:500

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-028-92
Standard Size100 µl
SpeciesMouse
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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