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Vasohibin [Cys0] (286-299) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-96 100 l $450.00

Image   

Protocol for Immunohistochemistry:

 Tissue Sample

 Human placenta tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Vasohibin (286-299) (Human) Antiserum (Catalog No.:H-002-96)

 Optimal Dilution

 1:500 (overnight at 4 oC)

 Secondary Antibody

 Goat anti-Rabbit IgG, HRP conjugated

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-96
Standard Size100 l
SequenceCys - Gly - Arg - Asp - Asp - Phe - Arg - Lys - Glu - Leu - Glu - Arg - His - Ala - Arg
SpeciesHuman
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3.
Cross ReactivityThis antibody is crossreactivity with human Vasohibin (286-299) at 100%. It was tested in immunohistochemistry in human placenta tissues. This antibody does not cross react with Human Vasohibib (336-365).
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