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H-005-06
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ANP, alpha (1-28) (Human, Porcine, Ovine, Canine) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-005-06
50 µl
$175.00
ANP (Atrial Natriuretic Peptide)
Normal Plasma Peptide/Protein Levels
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Rat or Mouse heart tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-ANP-alpha (1-28) (Human) (Catalog No.:H-005-06)
Optimal Dilution
1:500
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (sigma), 30min
Counterstained
Hematoxylin, 30Sec
Catalog #
H-005-06
Family
ANP
Standard Size
50 µl
Sequence
Ser - Leu - Arg - Arg - Ser - Ser - Cys - Phe - Gly - Gly - Arg - Met - Asp - Arg - Ile - Gly - Ala - Gln - Ser - Gly - Leu - Gly - Cys - Asn - Ser - Phe - Arg - Tyr
Disulfide Bridge:
Cys7 - Cys23
Species
Human, Canine, Ovine, Porcine
Host
Rabbit
Reconstitution
For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of rabbit anti- Atrial Natriuretic Polypeptide
(
a
-ANP) (1-28) (Human, Canine, Porcine, Bovine) Serum in the lyophilized form.
Recommended Dilution Factor
Immunofluorescence- 1:200
ABC- 1:500
Cross Reactivity
Peptide
% Cross-reactivity
a
-ANP (1-28) (Human, Ovine, Canine)
100
b
-ANP (Human
100
Urodilatin
100
ANP (Rat)
0.23
BNP-32 (Human)
0.024
Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)
0.01
CNP (24-53)
0.009
BNP-26 (Porcine)
0.006
BNP-45 (Rat)
0
Alphabet_A_
Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
Biondo et al. Immunohistochemistry of atrial and brain natriuretic peptides in control cats and cats with hypertrophic cardiomyopathy.
Vet Pathol. 2003 Sep;40(5):501-6.
Bialik et al. Evaluation of atrial natriuretic peptide and brain natriuretic peptide in atrial granules of rats with experimental congestive heart failure.
J Histochem Cytochem. 2001 Oct;49(10):1293-300.
Chen et al. The modulating actions of sulfonylurea on atrial natriuretic peptide release in experimental acute heart failure.
Eur J Heart Fail. 2000 Mar;2(1):33-40.
Lai et al. Up-regulation of adrenal cortical and medullary atrial natriuretic peptide and gene expression in rats with deoxycorticosterone acetate-salt treatment.
Endocrinology. 2000 Jan;141(1):325-32.
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