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Visfatin (400-450) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-003-84 100 l $280.00

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Protocol for Immunohistochemistry 
 Tissue Sample
 Human visceral white fat tissue, rat & mouse liver tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative Control No primary antibody (pre-immuno serum)
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Visfatin (400-450) (Human) Antiserum (Catalog No.:H-003-84)
 Optimal Dilution 1:200-500 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate

DAB (Sigma), 3 min

 CounterstainedHematoxylin, 30 Sec
Catalog #H-003-84
Standard Size100 l
SequenceLys - Cys - Ser - Tyr - Val - Val - Thr - Asn - Gly - Leu - Gly - Ile - Asn - Val - Phe - Lys - Asp - Pro - Val - Ala - Asp - Pro - Asn - Lys - Arg - Ser - Lys - Lys - Gly - Arg - Leu - Ser - Leu - His - Arg - Thr - Pro - Ala - Gly - Asn - Phe - Val - Thr - Leu - Glu - Glu - Gly - Lys - Gly - Asp - Leu
SpeciesHuman
HostRabbit
ReconstitutionLyophilized antiserum are stable for twelve months from date of receipt when stored at -20C to -70C. For best and reproducible results, reconstitute with 100l of distilled water for the equivalent of undiluted antiserum, immediately before use. Upon reconstitution, the antibody can be stored at 2Please click here or click the reference tab on the top to see more articles that use this product.
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