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H-003-84
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Visfatin (400-450) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-003-84
100 µl
$280.00
Visfatin
Normal Plasma Peptide/Protein Levels
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Human visceral white fat tissue, rat & mouse liver tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative Control
No primary antibody (pre-immuno serum)
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Visfatin (400-450) (Human) Antiserum (Catalog No.:H-003-84)
Optimal Dilution
1:200-500 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-003-84
Standard Size
100 µl
Sequence
Lys - Cys - Ser - Tyr - Val - Val - Thr - Asn - Gly - Leu - Gly - Ile - Asn - Val - Phe - Lys - Asp - Pro - Val - Ala - Asp - Pro - Asn - Lys - Arg - Ser - Lys - Lys - Gly - Arg - Leu - Ser - Leu - His - Arg - Thr - Pro - Ala - Gly - Asn - Phe - Val - Thr - Leu - Glu - Glu - Gly - Lys - Gly - Asp - Leu
Species
Human
Host
Rabbit
Reconstitution
Lyophilized antiserum are stable for twelve months from date of receipt when stored at -20°C to -70°C. For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Upon reconstitution, the antibody can be stored at 2Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
de Boer et al. Plasma levels of PBEF/Nampt/visfatin are decreased in patients with liver cirrhosis.
Am J Physiol Gastrointest Liver Physiol. 2009 Feb;296(2):G196-201.
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