CART (55-102) (Human) - Antibody for Immunohistochemistry

Catalog #

H-003-60

Standard Size

50 µl

Sequence

Val - Pro - Ile - Tyr - Glu - Lys - Lys - Tyr - Gly - Gln - Val - Pro - Met - Cys - Asp - Ala - Gly - Glu - Gln - Cys - Ala - Val - Arg - Lys - Gly - Ala - Arg - Ile - Gly - Lys - Leu - Cys - Asp - Cys - Pro - Arg - Gly - Thr - Ser - Cys - Asn - Ser - Phe - Leu - Leu - Lys - Cys - Leu [Disulfide bonds between Cys68 - Cys86, Cys74 - Cys94 and Cys88 - Cys101]

Species

Human

Host

Rabbit

Reconstitution

For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of rabbit anti-CART (55-102) (Human) serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

Cross Reactivity

*Peptide*	*% Cross-reactivity*
CART (55-102) (Human)	100
CART (55-102) (Rat)	100
Agouti-Related Protein (83-132)-NH₂ (Human)	2.3
Leptin (Human)	0
Neuropeptide (Human, Rat)	0
a-MSH	0
Orexin A (Human, Mouse, Rat)	0
Orexin B (Human)	0

Alphabet_C_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Yanik et al. The Leu34Phe ProCART mutation leads to cocaine- and amphetamine-regulated transcript (CART) deficiency: a possible cause for obesity in humans.
Endocrinology. 2006 Jan;147(1):39-43.

Dun et al. Cocaine- and amphetamine-regulated transcript peptide in the rat epididymis: an immunohistochemical and electrophysiological study.
Biol Reprod. 2000 Nov;63(5):1518-24.