MSH, Gamma2 (Human, Rat, Mouse) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human ventral and lateral hypothalamus
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	Target retrieval 25 min (Steam)
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-MSH-gamma Serum (Catalog No.:H-043-16)
Optimal Dilution	1:2000 (1hour at RT)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-043-16

Standard Size

50 µl

Sequence

Tyr - Val - Met - Gly - His - Phe - Arg - Trp - Asp - Arg - Phe - Gly

Species

Human, Rat, Mouse

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti-g MSH Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

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