中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站
www.phoenixpeptide.com
Top
»
Catalog
»
Antibodies
»
For Immunohistochemistry
»
H-071-22
English Version
|
My Account
|
联系我们
|
China
多肽
标记多肽
多肽激素文库
抗体
免疫组化抗体
纯化免疫球蛋白
抗体标记
免疫试剂盒
生物标志物阵列
多肽样品检测
自定义肽链合成及GMP
产品目录索取
样品准备
提问和解答
Urotensin II, Prepro (71-84) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-071-22
100 µl
$450.00
Urotensin II
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Mouse & rat brain tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-urotensin II, Prepro (71-84) (Human) Serum (Catalog No.:H-071-22)
Optimal Dilution
1:500 (over night at 4°C)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Western Blot Analysis of Prepro Urotensin II in rat brain tissue
Tissue Sample
Rat brain tissue homogenate
Electrophoresis
15% Tricine-PAGE gel
Negative control
No primary antibody
Blocking
1% BSA in TBST or 5% dry milk in TBST
Primary Antibody
Rabbit Anti-Urotensin, Prepro (71-84)(Human) Antiserum (Catalog No.:H-071-22)
Optimal Dilution
1:500 (4 hour at RT, or overnight at 4
0
C)
Secondary Antibody
Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
Detection system
HRP
Substrate
SuperSignal Chemiluminescent Substrate
Catalog #
H-071-22
Standard Size
100 µl
Sequence
Ala - Asp - Ser - Ser - Thr - Asn - Ile - Phe - Asn - Pro - Arg - Gly - Asn - Leu
Species
Human
Host
Rabbit
Reconstitution
For consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 100µl of rabbit anti-Urotensin II, Prepro (71-84) (Human) serum in the lyophilized form.
Recommended Dilution Factor
Direct ELISA: 1:3200
Immonohistochemistry (ABC) 1:500
Alphabet_U_
分类搜索
CHOOSE CATEGORIES
• ALL CATEGORIES
• Peptides
• Labeled Peptides
Fluorescent
Radioactive
Biotin
• Peptide Libraries
• Antibodies
For Immunohistochemistry
Purified lgG
Labeled Antibodies
• Kits
RIA
ELISA/EIA
Western Blot
Dot Blot
• Biomarker Array
Cardiovascular
Obesity
关键字搜索
按字母搜索
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Friday 04 October, 2024
75605871 requests since Wednesday 05 April, 2006
Copyright © 2024
PHOENIX BIOTECH