CCK (26-33) (Non-Sulfated) (Human, Rat, Mouse, Canine, Bovine)

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Protocol for Immunohistochemistry:
Tissue Sample	Rat intestines / Human colon & colon cancer tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit anti-CCK (26-33) (H,R,M) antibody (Cat. No.: H-069-04)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-069-04

Standard Size

50 µl

Sequence

Asp - Tyr - Met - Gly - Trp - Met - Asp - Phe - NH2

Species

Human, Rat, Mouse, Canine

Host

Rabbit

Reconstitution

For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

This vial contains 50µl of Rabbit Anti- Cholecystokinin Octapeptide (CCK 26-33) Serum in the lyophilized form.

Recommended Dilution Factor

Immunofluorescence: 1:200
PAP or ABC: 1:500

Cross Reactivity

*Peptide*	*% Cross-reactivity*
CCK 26-33 (Non-Sulfated)	100
CCK-33 (Porcine)	100
Gastrin-1 (Human)	100
Big Gastrin-1 (Human)	100
CCK 26-33 (Sulfated)	78
CCK 27-33	63
CCK 30-33	14
Pancreatic Polypeptide (Human)	0
VIP (Human, Porcine, Rat)	0

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Please click here or click the reference tab on the top to see more articles that use this product.

Links to publications that use this antibody:

Liu et al. Central expressions of ghrelin and cholecystokinin in rats with gastric electrical stimulation.
Obes Surg. 2008 Jan;18(1):109-14.

Rowniak et al. Somatostatin-like immunoreactivity in the amygdala of the pig
Folia Histochem Cytobiol. 2008;46(2):229-38.

Haeberle et al. Molecular profiling reveals synaptic release machinery in Merkel cells.
Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14503-8.