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C19orf10 / MYDGF Precursor (32-98)-amide (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-056-60 50 l $450.00

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Protocol for Immunohistochemistry
Tissue Sample Human Placenta
Fixative 4% Paraformaldehyde 8 hours
Embedding Paraffin
Negative control No primary antibody
Pretreatment  Intact 
Blocking 
 10% Normal Goat Serum (37 °C, 60 min)
Primary Antibody MYDGF precursor (32-98)-amide (Human)(Cat No.:H-056-60)
Optimal Dilution 1: 100 (1hour at 37 °C and overnight 4 °C)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (Vector Laboratory) (37 °C, 60 min)
Amplification ABC (Vector Laboratory) (30 min)
Detection system HRP
Substrate DAB (Vector Laboratory), 2 min
Counterstained Hematoxylin, 30 Sec
Catalog #H-056-60
Standard Size50 l
SpeciesHuman
HostRabbit
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