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SCNH2 / Selective Cutting Apelin-36 (1-16)-amide (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-057-36 50 l $450.00

Image

Protocol for Immunohistochemistry
Tissue Sample Human Placenta
Fixative 4% Paraformaldehyde 8 hours
Embedding Paraffin
Negative control No primary antibody
Pretreatment  Intact 
Blocking 
 2% Normal Goat Serum (37 °C, 60 min)
Primary Antibody SCNH2 / Selective Cutting Apelin-36(1-16)-amide (Human) (Catalog No.:H-057-36)
Optimal Dilution 1: 100 (1hour at 37 °C and overnight 4 °C)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (Vector Laboratory) (37 °C, 60 min)
Amplification ABC (Vector Laboratory) (30 min)
Detection system HRP
Substrate DAB (Vector Laboratory), 3 min
Counterstained Hematoxylin, 30 Sec

 

Catalog #H-057-36
Standard Size50 l
SpeciesHuman
HostRabbit
Recommended Dilution Factor 1: 100 (1 hour at 37 °C and overnight 4 °C)
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