Urotensin II, Prepro (21-40) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human placenta
Fixative	10% Formalin
Embedding	Paraffin
Negative control	Pre-immuno serum
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Urotensin II, Prepro (21-40) (Human) Serum (Catalog No.:H-071-24)
Optimal Dilution	1:500 (over night at 4°C)
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	ABC (Vector) (1:400, 30 min)
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-071-24

Standard Size

100 µl

Sequence

Leu - Pro - Leu - Leu - Asp - Ser - Arg - Glu - Ile - Ser - Phe - Gln - Leu - Ser - Ala - Pro - His - Glu - Asp - Ala

Species

Human

Reconstitution

Reconstitute with 100 ul distilled water.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

1: 500 (immunohistochemistry)
1: 3200 (indirect ELISA)

Cross Reactivity

This antibody is crossreactivity with human Urotensin II, Prepro (21-40). It was tested in human placenta tissues in immunohistochemistry

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