中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站 www.phoenixpeptide.com
PHOENIX PHARMACEUTICALS, INC. TOP HOME PAGE
TopCatalogAntibodiesFor ImmunohistochemistryH-071-22 English Version | My Account | 联系我们 | China



 多肽



 标记多肽 



 多肽激素文库



 抗体 
   免疫组化抗体
   纯化免疫球蛋白 
   抗体标记 



 免疫试剂盒 



 生物标志物阵列 



 多肽样品检测



 自定义肽链合成及GMP



 产品目录索取



 样品准备



 提问和解答


Urotensin II, Prepro (71-84) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-071-22 100 Ál $450.00

Image

    

Protocol for Immunohistochemistry
 Tissue Sample Mouse & rat brain tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-urotensin II, Prepro (71-84) (Human) Serum (Catalog No.:H-071-22)
 Optimal Dilution 1:500 (over night at 4°C)
 Secondary Antibody  Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec


Western Blot Analysis of Prepro Urotensin II in rat brain tissue
 Tissue Sample Rat brain tissue homogenate
 Electrophoresis 15% Tricine-PAGE gel
 Negative control No primary antibody
 Blocking 1% BSA in TBST or 5% dry milk in TBST
 Primary Antibody Rabbit Anti-Urotensin, Prepro (71-84)(Human) Antiserum (Catalog No.:H-071-22)
 Optimal Dilution 1:500 (4 hour at RT, or overnight at 4 0 C)
 Secondary Antibody Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection system HRP
 Substrate SuperSignal Chemiluminescent Substrate
Catalog #H-071-22
Standard Size100 Ál
SequenceAla - Asp - Ser - Ser - Thr - Asn - Ile - Phe - Asn - Pro - Arg - Gly - Asn - Leu
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100Ál of distilled water for the equivalent of undiluted antiserum immediately before use.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti-Urotensin II, Prepro (71-84) (Human) serum in the lyophilized form.
Recommended Dilution FactorDirect ELISA: 1:3200
Immonohistochemistry (ABC) 1:500
Alphabet_U_
分类搜索
关键字搜索
按字母搜索
A B C D E F G H I J K L M N
O P Q R S T U V W X Y Z

Copyright ę 2023 PHOENIX BIOTECH