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Urotensin II, Prepro (41-68) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-071-23 100 Ál $450.00

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 Protocol for Immunohistochemistry:
 Tissue Sample Human placenta,  Mouse & rat brain tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-urotensin II, Prepro (41-68) (Human) Serum (Catalog No.:H-071-23)
 Optimal Dilution 1:500 (over night at 4°C)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec

Catalog #H-071-23
Standard Size100 Ál
SequenceAla - Asp - Ser - Ser - Thr - Asn - Ile - Phe - Asn - Pro - Arg - Gly - Asn - Leu
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100Ál of distilled water for the equivalent of undiluted antiserum immediately before use.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti-Urotensin II, Prepro (41-68) (Human) serum in the lyophilized form.
Recommended Dilution FactorDirect ELISA: 1:3200
Immonohistochemistry (ABC) 1:500
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