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Insulin-1 C-Peptide (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-035-53 100 µl $295.00

Image    
Protocol for Immunohistochemistry: 
 Tissue Sample Human Pancreas Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-C-Peptide(Mouse) Antibody (Catalog No.:H-035-53)
 Optimal Dilution 1: 500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-035-53
Standard Size100 µl
SequenceGlu - Val - Glu - Asp - Pro - Gln - Val - Glu - Gln - Leu - Glu - Leu - Gly - Gly - Ser - Pro - Gly - Asp - Leu - Gln - Thr - Leu - Ala - Leu - Glu - Val - Ala - Arg - Gln
SpeciesMouse
HostRabbit
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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