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H-010-40
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Adrenomedullin-5 (Porcine) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-010-40
100 µl
$250.00
Adrenomedullin
Normal Plasma Peptide/Protein Levels
Adrenomedullin-5 Porcine
Antibody Production & Evaluation
Image
Protocol for
Immunohistochemistry
Tissue Sample
Human Placenta Tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No Primary Antibody
Pretreatment
N/A
Blocking
2% Normal Goat Serum, 3% H
2
O
2
Primary Antibody
Rabbit anti-Adrenomedullin-5 (Porcine) Antiserum (Cat. No.: H-010-40)
Optimal Dilution
1:500
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 sec
Catalog #
H-010-40
Family
Adrenomedullin
Standard Size
100 µl
Sequence
His - Gln - Val - Ser - Leu - Lys - Ser - Gly - Arg - Leu - Cys - Ser - Leu - Gly - Thr - Cys - Gln - Thr - His - Arg - Leu - Pro - Glu - Ile - Ile - Tyr - Trp - Leu - Arg - Ser - Ala - Ser - Thr - Lys - Glu - Leu - Ser - Gly - Lys - Ala - Gly - Arg - Lys - Pro - Gln - Asp - Pro - Tyr - Ser - Tyr - NH2 Disulfide bridge between Cys11 & Cys16
Species
Porcine
Host
Rabbit
Immunogen
Adrenomedullin-5 (Porcine)
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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