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Augurin, Prepro (71-107) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-012-22 100 l $250.00

Image

     

Protocol for Immunohistochemistry
 Tissue Sample

Human pancreas tissues

 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-Augurin, Prepro (71-107) (H) Antiserum (Catalog No.: H-012-22)

 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec

Lane #1: Porcine Pancreas Extracts
Lane #2: Bovine Hypothalamus Extracts
Lane #3: Bovine Pituitary Extracts
Lane #4: Porcine Liver Extracts
The major band in the porcine pancreas is 14.2 kDa augurin, but the bovine hypothalamus and pituitary show augurin derived peptides with the size at 14.1, 9.6 kDa and 6.5 kDa which can be detected by anti-augurin (71-107) antibody.
Catalog #H-012-22
Familyaugurin
Standard Size100 l
SequenceGlu - Leu - Trp - Asp - Arg - Thr - Arg - Pro - Glu - Val - Gln - Gln - Trp - Tyr - Gln - Gln - Phe - Leu - Tyr - Met - Gly - Phe - Asp - Glu - Ala - Lys - Phe - Glu - Asp - Asp - Ile - Thr - Tyr - Trp - Leu - Asn - Arg
SpeciesHuman
Hostrabbit
ImmunogenAugurin, prepro (71-107) (H)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry - 1:500
Alphabet_A_

%012-22%;%012-23%;%012-24%

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