Protocol for Immunohistochemistry:
Tissue Sample
Human pancreas tissue
Fixative
10% formalin
Embedding
Paraffin
Negative Control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-TRB-3 (314-349) (Rat) Antibody (Catalog No.:H-003-21)
Optimal Dilution
1:200
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec