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Alarin (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-026-34 100 ul $350.00

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Protocol for Immunohistochemistry
 Tissue SampleMouse/Rat Brain Tissue
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-Alarin (Human) Antibody (Catalog No.: H-026-34)

 Optimal Dilution

1:200, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-026-34
FamilyAlarin
Standard Size100 ul
SequenceAla - Pro - Ala - His - Arg - Ser - Ser - Thr - Phe - Pro - Lys - Trp - Val - Thr - Lys - Thr - Glu - Arg - Gly - Arg - Gln - Pro - Leu - Arg - Ser
SpeciesHuman
HostRabbit
ImmunogenHuman Alarin
ReconstitutionFor best and reproducible results, reconstitute with 100 l of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry - 1:500
Alphabet_A_

%026-34%;%026-31%;%026-32%;%026-33%

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