Spexin, Prepro (36-58) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human stomach tissues and stomach cancer tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Spexin, Prepro (36-58) (H) Antiserum (Catalog No.: H-023-82)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-023-82

Family

Spexin

Standard Size

100 µl

Sequence

Asn - Trp - Thr - Pro - Gln - Ala - Net - Leu - Tyr - Leu - Lys - Gly - Ala - Gln - Gly - Arg - Arg - Phe - Ile - Ser - Asp - Gln - Ser

Species

Human

Host

Rabbit

Immunogen

Spexin, Prepro (36-58) (Human)

Reconstitution

100 ul

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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