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H-010-28
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Adrenomedullin (1-44) (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-010-28
100 µl
$370.00
Adrenomedullin
Normal Plasma Peptide/Protein Levels
Adrenomedullin-5 Porcine
Antibody Production & Evaluation
Image
Protocol for
Immunohistochemistry
Tissue Sample
Human/Rat Lung and Lunch Cancer Tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No Primary Antibody
Pretreatment
Target Retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum, 3% H
2
O
2
Primary Antibody
Anti-Adrenomedullin (1-44) (Human) Antibody (Catalog No.: H-010-28)
Optimal Dilution
1:500 (1hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 sec
Catalog #
H-010-28
Family
Adrenomedullin
Standard Size
100 µl
Sequence
Tyr - Arg - Gln - Ser - Met - Asn - Asn - Phe - Gln - Gly - Leu - Arg - Ser - Phe - Gly - Cys - Arg - Phe - Gly - Thr - Cys - Thr - Val - Gln - Lys - Leu - Ala - His - Gln - Ile - Tyr - Gln - Phe - Thr - Asp - Lys - Asp - Lys - Asp - Asn - Val - Ala - Pro - Arg, Disulfide Bridge: Cys16 - Cys21
Species
Human
Host
Rabbit
Immunogen
Adrenomedullin (1-44) (Human)
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_A_
%010-28%;%010-37%
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