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H-010-37
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Adrenomedullin-Gly (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-010-37
100 µl
$475.00
Adrenomedullin
Normal Plasma Peptide/Protein Levels
Adrenomedullin-5 Porcine
Antibody Production & Evaluation
Image
Protocol for
Immunohistochemistry
Tissue Sample
Human Lung Cancer & Pancreatic Tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No Primary Antibody
Pretreatment
Target Retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum, 3% H
2
O
2
Primary Antibody
Anti-Adrenomedullin-Gly (Human) Antibody (Catalog No.: H-010-37)
Optimal Dilution
1:500 (1hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
Streptavidin-HRP (Vector), 1:400, 30 min
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 sec
Catalog #
H-010-37
Family
Adrenomedullin
Standard Size
100 µl
Sequence
Tyr - Arg - Gln - Ser - Met - Asn - Asn - Phe - Gln - Gly - Leu - Arg - Ser - Phe - Gly - Cys - Arg - Phe - Gly - Thr - Cys - Thr - Val - Gln - Lys - Leu - Ala - His - Gln - Ile - Tyr - Gln - Phe - Thr - Asp - Lys - Asp - Lys - Asp - Asn - Val - Ala - Pro - Arg - Ser - Lys - Ile - Ser - Pro - Gln - Gly - Tyr - Gly Disulfide Bridge: Cys16 - Cys21
Species
Human
Host
Rabbit
Immunogen
Adrenomedullin-Gly
Reconstitution
For best and reproducible results, reconstitute with 100 µl of distilled water for the equivalent of undiluted antiserum. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Appearance
lyophilized form.
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