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Vasostatin I (17-76) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-053-35 100 l $450.00

Image    
Protocol for Immunohistochemistry:
 Tissue Sample Human adrenal medullary tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment N/A
 Blocking

 3% H2O2, 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-vasostatin I (17-76) (Human) (Cat. No.: H-053-35)

 Optimal Dilution 1:500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection system HRP
 Substrate DAB (sigma), 30min
 Counterstained Hematoxylin, 30Sec
Catalog #H-053-35
FamilyPlease call for status of this item
Standard Size100 l
SequenceCys - Ile - Val - Glu - Val - Ile - Ser - Asp - Thr - Leu - Ser - Lys - Pro - Ser - Pro - Met - Pro - Val - Ser - Gln - Glu - Cys - Phe - Glu - Thr - Leu - Arg - Gly - Asp - Glu - Arg - Ile - Leu - Ser - Ile - Leu - Arg - His - Gln - Asn - Leu - Leu - Lys - Glu - Leu - Gln - Asp - Leu - Ala - Leu - Gln - Gly - Ala - Lys - Glu - Arg - Ala - His - Gln - Gln
SpeciesHuman
HostRabbit
Immunogenhuman Vasostatin (17-76)
Reconstitution100 ul
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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