Vasostatin I (17-76) (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample	Human adrenal medullary tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit Anti-vasostatin I (17-76) (Human) (Cat. No.: H-053-35)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (sigma), 30min
Counterstained	Hematoxylin, 30Sec

Catalog #

H-053-35

Family

Please call for status of this item

Standard Size

100 µl

Sequence

Cys - Ile - Val - Glu - Val - Ile - Ser - Asp - Thr - Leu - Ser - Lys - Pro - Ser - Pro - Met - Pro - Val - Ser - Gln - Glu - Cys - Phe - Glu - Thr - Leu - Arg - Gly - Asp - Glu - Arg - Ile - Leu - Ser - Ile - Leu - Arg - His - Gln - Asn - Leu - Leu - Lys - Glu - Leu - Gln - Asp - Leu - Ala - Leu - Gln - Gly - Ala - Lys - Glu - Arg - Ala - His - Gln - Gln

Species

Human

Host

Rabbit

Immunogen

human Vasostatin (17-76)

Reconstitution

100 ul

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Alphabet_V_