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H-050-50
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TRPM8 / CMR-1 (656-680) (Rat) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-050-50
50 µl
$450.00
Icilin (AG-3-5)
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Rat ganglia
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
Target retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti- CMR-1 (656-680) (Rat) Antiserum (Catalog No.:H-050-50)
Optimal Dilution
1:100 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-050-50
Standard Size
50 µl
Sequence
Cys - Leu - Glu - Leu - Ala - Val - Glu - Ala - Thr - Asp - Gln - His - Phe - Ile - Ala - Gln - Pro - Gly - Val - Gln - Asn - Phe - Leu - Ser - Lys
Species
Rat
Host
Rabbit
Reconstitution
For best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of rabbit anti- CMR-1 (656-680) (Rat) serum in the lyophilized form.
Alphabet_T, C_
Please click
here
or click the reference tab on the top to see more articles that use this product.
Links to publications that use this antibody:
Alvarado et al. TRPM8 Axonal Expression is Decreased in Painful Human Teeth with Irreversible Pulpitis and Cold Hyperalgesia
J Endod. 2007 Oct;33(10):1167-71.
Stein et al. Cool (TRPM8) and hot (TRPV1) receptors in the bladder and male genital tract.
J Urol. 2004 Sep;172(3):1175-8.
Facer et al. Differential expression of the capsaicin receptor TRPV1 and related novel receptors TRPV3, TRPV4 and TRPM8 in normal human tissues and changes in traumatic and diabetic neuropathy
BMC Neurol. 2007 May 23;7:11.
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