Protocol for Immunohistochemistry:
Tissue Sample
Mouse & rat brain tissue
Fixative
10% formalin
Embedding
Paraffin
Negative Control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-GPR-30 (351-375) (Human) Serum (Catalog No.:H-002-84)
Optimal Dilution
1:500
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection System
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec