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Bombinakinin-GAP - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-009-38 50 l $450.00

Image

Rat hypothalamus tissue was stained by
Bombinakinin-GAP Antiserum

Rat hypothalamus tissue was stained by
Bombinakinin-GAP Antiserum


Protocol for Immunohistochemistry
Tissue Sample Rat hypothalamus , cortex and skin
Fixative 10% Formalin
Embedding Paraffin
Negative control No primary antibody
Pretreatment Target retrieval 25 min (Steam
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti- Bombinakinin-GAP Antiserum (Catalog No.:H- 009-38)
Optimal Dilution 1:2000 (1hour at RT)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification  ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec
Catalog #H-009-38
Standard Size50 l
SequenceAsp - Met - Tyr - Glu - Ile - Lys - Gln - Tyr - Lys - Thr - Ala - His - Gly - Arg - Pro - Pro - Ile - Cys - Ala - Pro - Gly - Glu - Gln - Cys - Pro - Ile - Trp - Val - NH2 (Disulfide bridge between Cys18 and Cys24 )
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50l of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit anti- Bombinakinin-GAP serum in the lyophilized form.
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