中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站 www.phoenixpeptide.com
PHOENIX PHARMACEUTICALS, INC. TOP HOME PAGE
Top Catalog Antibodies For Immunohistochemistry H-009-01 English Version | My Account | 联系我们 | China



 多肽



 标记多肽 



 多肽激素文库



 抗体 
   免疫组化抗体
   纯化免疫球蛋白 
   抗体标记 



 免疫试剂盒 



 生物标志物阵列 



 多肽样品检测



 自定义肽链合成及GMP



 产品目录索取



 样品准备



 提问和解答


Bradykinin (Human, Rat, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-009-01 50 l $175.00

Image

    

Protocol for Immunohistochemistry
 Tissue Sample Rat Spinal Cord
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No Primary Antibody
 PretreatmentTarget Retrieval 25 min (Steam)
 Blocking2% Normal Goat Serum
 Primary AntibodyRabbit Anti-Bradykinin Antiserum (Catalog No.:H-009-01)
 Optimal Dilution1:200 (1hour at RT)
 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 AmplificationABC (Vector) (1:400, 30 min)
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin, 30 sec
Catalog #H-009-01
Standard Size50 l
SequenceArg - Pro - Pro - Gly - Phe - Ser - Pro - Phe - Arg
SpeciesHuman, Rat, Mouse
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50l of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit Anti-Bradykinin Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC: 1:200
Immunohistochemistry: 1:500
Cross Reactivity
Peptide % Cross-reactivity 
Bradykinin 100
Lys-Bradykinin100 
Biotinyl-Bradykinin 100 
[Tyr0]-Bradykinin100 
[Des-Arg9]-Bradykinin 
Met-Enkephalin 
Substance P
Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)0
TRH
Alphabet_B_
分类搜索
关键字搜索
按字母搜索
A B C D E F G H I J K L M N
O P Q R S T U V W X Y Z

Copyright 2019 PHOENIX BIOTECH