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H-009-01
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Bradykinin (Human, Rat, Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-009-01
50 µl
$175.00
Bradykinin and Related Peptides
Normal Plasma Peptide/Protein Levels
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Rat Spinal Cord
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No Primary Antibody
Pretreatment
Target Retrieval 25 min (Steam)
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Bradykinin Antiserum (Catalog No.:H-009-01)
Optimal Dilution
1:200 (1hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 sec
Catalog #
H-009-01
Standard Size
50 µl
Sequence
Arg - Pro - Pro - Gly - Phe - Ser - Pro - Phe - Arg
Species
Human, Rat, Mouse
Host
Rabbit
Reconstitution
For consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
This vial contains 50µl of rabbit Anti-Bradykinin Serum in the lyophilized form.
Recommended Dilution Factor
Immunofluorescence: 1:200
PAP or ABC: 1:200
Immunohistochemistry: 1:500
Cross Reactivity
Peptide
% Cross-reactivity
Bradykinin
100
Lys-Bradykinin
100
Biotinyl-Bradykinin
100
[Tyr0]-Bradykinin
100
[Des-Arg9]-Bradykinin
0
Met-Enkephalin
0
Substance P
0
Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine)
0
TRH
0
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