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H-008-29
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Autotaxin (369-391) (Human, Mouse) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-008-29
100 µl
$450.00
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry
Tissue Sample
Rat & Mouse Kidney, Rat Liver
Fixative
10% Formalin
Embedding
Paraffin
Negative control
Pre-immuno serum
Pretreatment
Intact
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti-Autotaxin (369-399) (Human, Mouse) Serum (Catalog No.:H-008-29)
Optimal Dilution
1:250~500 (1hour at RT)
Secondary Antibody
Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin
Catalog #
H-008-29
Standard Size
100 µl
Sequence
Thr - Glu - Phe - Leu - Ser - Asn - Tyr - Leu - Thr - Asn - Val - Asp - Asp - Ile - Thr - Leu - Val - Pro - Gly - Thr - Leu - Gly - Arg
Species
Human, Mouse
Reconstitution
Reconstitute with 100 ul distill water.
Storage Condition
Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content
100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3.
Recommended Dilution Factor
Indirect ELISA: 1: 6400
Immunohistochemistry:1: 250~ 500
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