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Autotaxin (369-391) (Human, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-008-29 100 l $450.00

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Protocol for Immunohistochemistry
 Tissue Sample Rat & Mouse Kidney, Rat Liver
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 PretreatmentIntact
 Blocking2% Normal Goat Serum
 Primary AntibodyRabbit Anti-Autotaxin (369-399) (Human, Mouse) Serum (Catalog No.:H-008-29)
 Optimal Dilution1:250~500 (1hour at RT)
 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400) 
 AmplificationABC (Vector) (1:400, 30 min)
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin
Catalog #H-008-29
Standard Size100 l
SequenceThr - Glu - Phe - Leu - Ser - Asn - Tyr - Leu - Thr - Asn - Val - Asp - Asp - Ile - Thr - Leu - Val - Pro - Gly - Thr - Leu - Gly - Arg
SpeciesHuman, Mouse
ReconstitutionReconstitute with 100 ul distill water.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3.
Recommended Dilution FactorIndirect ELISA: 1: 6400
Immunohistochemistry:1: 250~ 500
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